郑旺;高丽娜;李西成;张隆;田志;

• 1:河北省人民医院骨二科

摘要(Abstract)：

[目的]探讨在外源性生长因子(BMP-2)作用下,使用胶原蛋白膜包裹经体外培养扩增的骨髓基质干细胞(BMSCs)与藻酸钙凝胶形成的复合物修复骨缺损的可行性。[方法]选用成年新西兰白兔36只。手术造成两侧桡骨标准缺损后,左侧植入复合体,右侧空白对照。取自体BMSCs,经体外分离培养扩增后,与BMP-2、藻酸钙凝胶及胶原蛋白膜形成复合体修复骨缺损,于2、4、8、12周时行大体、X线片、免疫组织化学观察。[结果]整个过程中可见实验组缺损区新生骨组织增殖明显、持续时间较长,且无过量的结缔组织生长;X线早期可见连续性骨痂通过缺损区,分布均匀;免疫组织化学可见多个成骨中心,骨小梁排列有序,成熟骨替代完全,BMP分布持续时间长,且在新骨组织中分布范围大。[结论]复合体能修复骨缺损,并且能够阻挡周围软组织进入缺损区,为新骨生长提供空间;同时作为BMP的载体,能减少外溢,提高其疗效。

关键词(KeyWords)： 胶原蛋白膜;藻酸钙凝胶;骨髓基质细胞;骨形态发生蛋白-2;骨缺损

Abstract:

Keywords:

基金项目(Foundation): 河北省卫生厅科研基金项目(编号:20100194)

作者(Author): 郑旺;高丽娜;李西成;张隆;田志;

Email:

DOI:

参考文献(References)：

• [1]Dallari D,Fini M,Stagni C,et al.In vivo study on the healing ofbone defects treated with bone marrow stromal cells,platelet-rich-plasma,and freeze-dried bone allografts,alone and in combination[J].J Orthop Res,2006,5:877-888.
• [2]Tomiyama K,Murase N,Stolz DB,et al.Characterization of trans-planted green fluorescent protein and bone marrow cells into adiposetissue[J].Stem Cells,2008,2:330-338.
• [3]陈润良,刘磊,田卫东,等.间充质干细胞复合藻酸盐凝胶体内成软骨分泌Ⅱ型胶原的代谢功能特征[J].中国组织工程研究与临床康复,2007,10:1815-1817.
• [4]刘磊,陈润良,田卫东,等.大鼠骨髓间充质干细胞复合藻酸盐体内异位成软骨的初步研究[J].华西口腔医学杂志,2005,1:60-62.
• [5]Lin YF,Tang W,Wu L,et al.Bone regeneration by BMP-2 en-hancedadipose stem cells loading on alginate gel[J].HistochemCell Biol,2008,129:203-210.
• [6]Paige KT,Cima LC,Yaremehuk MJ,et al.Injectable cartilage[J].Plast Reconstr,1995,96:1390-1398.
• [7]杨志明,黄富国,秦廷武,等.生物衍生组织工程骨植骨的初步临床应用[J].中国修复重建外科杂志,2002,16:311-314.
• [8]Dvorakova J,Hruba A,Velebny V,et al.Isolation and characteriza-tion of mesenchymal stem cell population entrapped in bone marrowcollection sets[J].Cell Biol Int,2008,9:1116-1125.
• [9]盛辉,王洪复.骨髓基质细胞向成骨细胞诱导分化的研究进展[J].中国骨质疏松杂志,2004,1:98-102.
• [10]Yamada Y,Ueda M,Hibi H,et al.Translational research for inject-able tissue-engineered bone regeneration using mesenchymal stemcells and platelet-rich plasma:from basic research to clinical casestudy[J].Cell Transplant,2004,4:343-355.
• [11]王立春,刘明辉,张宏颖,等.骨形成蛋白2基因修饰的骨髓基质细胞与纤维蛋白复合物修复兔关节软骨缺损[J].中国修复重建外科杂志,2005,12:960-965.
• [12]Kilic E,Ceyhan T,Cetinkaya DU.Evaluation of differentiation po-tential of human bone marrow-derived mesenchymal stromal cells tocartilage and bone cells[J].Acta Orthop Traumatol Turc,2007,4:295-301.
• [13]Marolt D,Augst A,Freed LE,et al.Bone and cartilage tissue con-structs grown using human bone marrow stromal cells,silk scaffoldsand rotating bioreactors[J].Biomaterials,2006,36:6138-6149.
• [14]Zaky SH,Ottonello A,Strada P,et al.Platelet lysate favours in vitroexpansion of human bone marrow stromal cells for bone and carti-lage engineering[J].J Tissue Eng Regen Med,2008,8:472-481.
• [15]Kuroda R,Ishida K,Matsumoto T,et al.Treatment of a full-thick-ness articular cartilage defect in the femoral condyle of an athletewith autologous bone-marrow stromal cells[J].Osteoarthritis Carti-lage,2007,2:226-231.
• [16]Liu ZJ,Zhuge Y,Velazquez OC.Trafficking and differentiation ofmesenchymal stem cells[J].J Cell Biochem,2009,6:984-991.
• [17]El Tamer MK,Reis RL.Progenitor and stem cells for bone and carti-lage regeneration[J].J Tissue Eng Regen Med,2009,5:327-337.
• [18]高云静,刘新.羟基磷灰石和骨生长因子复合修复颌骨缺损的实验研究[J].中国实验诊断学,2003,6:493-494.
• [19]李文辉,侯筱魁,汤亭亭,等.藻酸钙复合材料构建组织工程化骨软骨的实验研究[J].中国矫形外科杂志,2004,7:517-519.
• [20]刘阳,谭最.组织工程血管支架研究进展[J].武汉大学学报医学版,2006,1:124-129.
• [21]Guehennec L,Layrolle P,Daculsi G.A review of bioceramics and fi-brin sealant[J].Eur Cell Mater,2004,8:1-11.