胶原蛋白膜包裹藻酸钙凝胶/骨髓基质细胞/BMP-2复合体修复兔桡骨缺损Collagen membrane wraps the complex of calcium alginate gelatin,bone marrow stromal cells and bone morphogenetic protein to repair radius defect of rabbits
郑旺;高丽娜;李西成;张隆;田志;
摘要(Abstract):
[目的]探讨在外源性生长因子(BMP-2)作用下,使用胶原蛋白膜包裹经体外培养扩增的骨髓基质干细胞(BMSCs)与藻酸钙凝胶形成的复合物修复骨缺损的可行性。[方法]选用成年新西兰白兔36只。手术造成两侧桡骨标准缺损后,左侧植入复合体,右侧空白对照。取自体BMSCs,经体外分离培养扩增后,与BMP-2、藻酸钙凝胶及胶原蛋白膜形成复合体修复骨缺损,于2、4、8、12周时行大体、X线片、免疫组织化学观察。[结果]整个过程中可见实验组缺损区新生骨组织增殖明显、持续时间较长,且无过量的结缔组织生长;X线早期可见连续性骨痂通过缺损区,分布均匀;免疫组织化学可见多个成骨中心,骨小梁排列有序,成熟骨替代完全,BMP分布持续时间长,且在新骨组织中分布范围大。[结论]复合体能修复骨缺损,并且能够阻挡周围软组织进入缺损区,为新骨生长提供空间;同时作为BMP的载体,能减少外溢,提高其疗效。
关键词(KeyWords): 胶原蛋白膜;藻酸钙凝胶;骨髓基质细胞;骨形态发生蛋白-2;骨缺损
基金项目(Foundation): 河北省卫生厅科研基金项目(编号:20100194)
作者(Author): 郑旺;高丽娜;李西成;张隆;田志;
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DOI:
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