褪黑素与全反式维甲酸共同作用对大鼠脊髓神经干细胞分化的影响Effects of melatonin and all-trans retinoic acids on the differentiation of spinal neural stem cells of embryo rat in vitro
龚凯;罗卓荆;禹晓东;褚尤标;
摘要(Abstract)：
[目的]研究全反式维甲酸(all-trans retinoic ac id,ATRA)与不同浓度的褪黑素(m elaton in,MT)共同作用对体外培养的胚胎大鼠脊髓神经干细胞(neural stem cells,NSCs)向神经元方向分化的影响。[方法]取孕14 d的胚胎大鼠脊髓,原代培养出脊髓NSCs,传4代后分别加入ATRA与不同浓度的MT组合。采用细胞计数结合免疫荧光检测法,观察NSCs细胞的生长增殖及分化状况,并作统计学分析。[结果]与对照组[(8.8±1)%]相比,单纯ATRA作用组神经元分化比率[(10.6±2%]无明显差异;单纯MT作用组100 mol/L[(24.8±2)%]与10 nmol/LMT[23.4±1%)]2组都能明显促进脊髓NSCs向神经元分化,2组间比较无明显差异;ATPA与100μmol/LMT共同作用组[(53.9±2)%],进一步提高了脊髓NSCs向神经元分化的比率;ATRA与10 nmol/LMT共同作用组[(26.7±2)%]也能促进脊髓NSCs向神经元方向分化(与对照组相比较),但其分化率与单一使用MT的2组比较无明显差异。[结论]单纯应用不同浓度的MT都促进脊髓源性的NSCs向神经元分化,但ATRA与不同浓度的MT联用对神经元分化率有着不同影响。
关键词(KeyWords)： 神经干细胞;褪黑素;全反式维甲酸;诱导分化;神经元
基金项目(Foundation): 国家“863”资助项目(2002AA216101)
作者(Author): 龚凯;罗卓荆;禹晓东;褚尤标;
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DOI:
参考文献(References)：
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