许红生;刘金钊;

• 1:青岛大学医学院附属医院关节外科

摘要(Abstract)：

间充质干细胞具有高度自我更新能力,能够分化为各种类型的细胞。近20年间充质细胞在软骨组织工程中得到应用,动物实验显示了良好的软骨修复潜能,成为软骨组织工程的理想的细胞来源。组织工程和干细胞技术已经确定为对软骨缺损有效的治疗方法,间充质干细胞有望在软骨组织工程中得到更广泛的应用。本文对间充质干细胞的特性和在组织工程中的应用进行了综述。

关键词(KeyWords)： 间充质干细胞;骨性关节炎;组织工程;自体软骨细胞移植术

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 许红生;刘金钊;

Email:

DOI:

参考文献(References)：

• [1]Peterson L,Brittberg M,Kiviranta I,et al.Autologous chondrocytetransplantation.Biomechanics and long-term durability[J].J SportsMed(Am),2002,30:2-12.
• [2]Schulze-Tanzil G,Mobasheri A,de Souza P,et al.Loss of chondrogen-lc potential in dedifferentiated chondrocytes correlates with deficientShc-Erk interaction and apoptosis[J].Osteoarthritis Cartilage,2004,12:448-458.
• [3]Csaki C,Matis U,Mobasheri A,et al.Chondrogenesis,osteogenesisand adipogenesis of canine mesenchymal stem cells:a biochemical,morphological and ultrastructural study[J].Histochem Cell Biol,2007,128:507-520.
• [4]Dominici M,Le Blanc K,Mueller I,et al.Minimal criteria for definingmultlpotent nesenchymal stromal cells.The international society forcellular therapy position statement[J].Cytotherapy,2006,8:315-317.
• [5]Watt SM,Contreras M.Stem cell medicine:umbilical cord blood andits stem cell potential[J].Semin Fetal Neonatal Med,2005,10:209-220.
• [6]Yamamoto N,Akamatsu H,Hasegawa S.Isolation of multipotent stemcells from mouse adipose tissue[J].J Dermatol Sci,2007,48:43-52.
• [7]Im GI,Shin YW,Lee KB.Do adipose tissuederived mesenchymalstem cells have the same osteogenic and chondrogenic potential asbone marrow-derived cells[J].Osteoarthritis Cartilage,2005,13:845-853.
• [8]Wagner W,Wein F,Seckinger A,et al.Comparative characteristicsof mesenchymal stem cells from human bone marrow,adipose tissue,and umbilical cord blood[J].Exp Hematol,2005,33:1402-1416.
• [9]Giovannini S,BrehmW,Mainil VP,et al.Multilineage differentiationpotential of equine blood-derived fibroblast-like cells[J].Differentia-tion,2008,76:118-129.
• [10]Louise A,McMahon A,Patrick J,et al.A comparison of the involve-ment ofβ38,ERK1/2and P13K in growth factor-induced chondro-genic differentiation of mesenchymal stem cells[J].Biochemical andBiophysical Research Communications,2008,368:990-995.
• [11]Jian H,Shen X,Liu I,et al.Smad3-dependent nuclear translocation ofbeta-catenin is required for TGF-betal-induced proliferation of bonemarrow-derived adult human mesenchymal stem cells[J].GenesDer,2006,20:666-674.
• [12]Keun HP,Kun N.Effect of growth factors on chondrogenic differentia-tion of rabbit mesenchymal cells embedded in injectable hydrogels[J].Journal of Bioscience and Bioengineering,2008,106:74-79.
• [13]Hansoo P,Johnna S,Temenoffb Y,et al.Injectable biodegradable hy-drogel composites for rabbit marrow mesenchymal stem cell andgrowth factor delivery for cartilage tissue engineering[J].Biomateri-als,2007,28:3217-3227.
• [14]Huang AH,Yeger-McKeever M,Steiny A,et al.Tensile properties ofengineered cartilage formed from chondrocyte-and MSC-laden hydro-gels[J].Osteoarthritis and Cartilage,2008,16:1074-1082.
• [15]Lorenz U,Hans P,Merkle L,et al.Insulin-like growth factor I relea-sing silk fibroin scaffolds induce chondrogenic differentiation of hu-man mesenchymal stem cells[J].Journal of Controlled Release,2008,127:12-21.
• [16]Derfoul A,Perkins GL,Hall DJ,et al.Glucocorticoids promote chon-drogenic differentiation of adult human mesenchymal stem cells byenhancing expression of cartilage extracellular matrix genes[J].StemCells,2006,24:1487-1495.
• [17]Knippenberg M,Helder MN,Zandieh DB,et al.Osteogenesis versuschondrogenesis by BMP-2 and BMP-7 in adipose stem cells[J].Biochem.Biophys Res Commun,2006,342:902-908.
• [18]Hanna T,Salla S,Teuvo H,et al.Impact of stromal cell compositionon BMP-induced chondrogenic differentiation of mouse bone marrowderived mesenchymal cells[J].Experimental Cell Research,2008,314:2400-2410.
• [19]王文良,张华亮,关静,等.壳聚糖/羟基磷灰石支架修复骨软骨缺损的实验研究[J].中国矫形外科杂志,2008,20:1579-1583.
• [20]Lφken S,Jakobsen RB,Aφroen A,et al.Bone marrow mesenchymalstem cells in a hyaluronan scaffold for treatment of an osteochondraldefect in a rabbit model[J].Knee Surg Sports Traumatol Arthrosc,2008,16:896-903.
• [21]Mrugala D,Bony C,Neves N,et al.Phenotypic and functional charac-terization of ovine mesenchymal stem cells:application to a cartilagedefect model[J].Ann Rheum Dis,2008,3:288-295.
• [22]Bernd W,Florian M,Schrimpf M,et al.Matrix-guided cartilage regen-eration in chondral defects[J].Biotechnol Appl Biochem,2008,16:1122.
• [23]Seung HH,Yun HK,Min SP,et al.Histological and biomechanicalproperties of regenerated articular cartilage using chondrogenic bonemarrow stromal cells with a PLGAscaffold in vivo[J].J Biomed Ma-ter Res A,2008,4:850-861.
• [24]Wakitani S,Imoto K,Yamamoto T,et al.Human autologous cultureexpanded bone marrowmesenchymal cell transplantation for repair ofcartilage defects in osteoarthritic knees[J].Osteoarthritis Cartilage,2002,10:199-206.
• [25]Wakitani S,Nawata M,Tensho K,et al.Repair of articular cartilagedefects in the patello-femoral joint with autologous bone marrowmes-enchymal cell transplantation:three case reports involving 9 defectsin 5 knees[J].J Tissue Eng Regen Med,2007,1:74-79.
• [26]Kuroda R,Ishida K,Matsumoto T,et al.Treatment of a full-thicknessarticular cartilage defect in the femoral condyle of an athlete with au-tologous bone-marrow stromal cells[J].Osteoarthritis Cartilage,2007,2:226-231.
• [27]Steck E,Fischer J,Lorenz H,et al.Mesenchymal stem cell differenti-ation in an experimental cartilage defect:restriction of hypertrophy tobone-close neocartilage[J].Stem Cells Dev,2008,2:112.
• [28]Hennig T,Lorenz H,Thiel A,et al.Potential of adipose tissue derivedstromal cells correlates with an altered TGFbeta receptor and BMPprofile and is overcome by BMP-6[J].J Cell Physiol,2007,3:682-691.