TGFβ_1和BMP_2对培养兔关节软骨细胞增殖和分化的作用Effects of Transforming Growth Factor β1 and Bone Morphogenetic Protein 2 (BMP_2) on Proliferation and Differentiation of Rabbit Articular Chondrocytes(RAC)Cultured in Vitro
杨物鹏,许建中,闫占明,吕福润,付万有,王春梅
摘要(Abstract)：
目的 :探讨TGFβ1和BMP2 对培养兔关节软骨细胞增殖和分化的影响 ,从而了解其在关节软骨损伤修复及骨关节炎 (OA)病理变化中的作用 ,方法 :采用光电镜、免疫细胞化学、液烁计数、流式细胞仪分析细胞周期等方法观察软骨细胞的超微结构和增殖、分化及其特异性的表达。结果 :TGFβ1显著增加了原代软骨细胞3 H TdR的掺入和使其进入S G2 /M期细胞比例增加。而BMP2 则对第 5代软骨细胞有明显的促增殖作用。结论 :TGFβ1能促进原代软骨细胞的增殖 ,但不能阻止其增殖能力随传代培养而减弱 ,而BMP2 则显著促进了第 5代软骨细胞的增殖 ,这可能与细胞的分化状态和细胞外基质 (ECM )、细胞骨架等有关
关键词(KeyWords)： 关节软骨细胞;骨形态发生蛋白2;转化生长因子β1;增殖;分化
基金项目(Foundation):
作者(Author): 杨物鹏,许建中,闫占明,吕福润,付万有,王春梅
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参考文献(References)：
- 〔1〕 DukepJ ,DauneEL ,MontufarSD .Studiesofchondrogene sisinrotatingsystem[J].JCellBionchem,1994,15(3):1～5.
- 〔2〕 VecantiCA ,CaoYL .Neo cartilagegeneratedfromchon drocytesisolatedfromoldhumancartilage[J].TransplantProc,1994,26(4):1069～1077.
- 〔3〕 NingWang,DonaldE ,Ingber.Controlofcytoskeletalme chanicalbyextracellularmatrix,cellshapeandmechanicaltension[J].BiophysicalJ ,1994,66:2181～2189.
- 〔4〕 PierreA ,ArthurS ,MartinL ,etal.Growthfactorrespon sivenessofhumanarticularchondrocytes:distinctprofilesinprimarychondrocyessubculturedchondrocytesandfibrob last[J].JCellPhysiol,1994,158:476～484.
- 〔5〕 K .Boumedine,Dovivien,M .macro,etal.Modulationofrab bitarticularchondrocytes(RAC) proliferationbyTGFβiso forms[J].CellProlif,1995,28:221～234.
- 〔6〕 DenisV ,FirancoiseR ,philippeG ,etal.Rabbitarticularchondrocytes(RAC )functionofcellcyclephase[J].ExpCellRes,1993,205:165～170.
- 〔7〕 OkeefeRJ ,PuzasJE ,BrandJS ,etal.EffectoftransforminggrowthfactorβonDNAsynthesisbygrowthplatechondro cytesmodulationbyfactorspresentinserum[J].CalcifTis sueInt,1988,43:352～358.
- 〔8〕 DenisV ,PhilippeG ,Emmanuel,etal.Differentiatedeffectoftransforminggrowthfactorβandepidermalgrowthfactoronthecellcycleofculturedrabbitarticularchondrocytes[J].JCellPhysiol,1990,143:534～545.
- 〔9〕 HildebrandA ,RomarisM ,RasmussenLM ,etal.Interationofthesmallinterstitialproteoglycansbiglycan,decorinandfibromodulinwithtransforming growthfactorβ[J ].BiochemJ ,1994,302(2):5275.
- 〔10〕司晓辉,杨连甲,金岩.转化生长因子β对骨形成蛋白2基因转染细胞生物学行为的影响[J].华西口腔医学杂志,1999,17(4):321～323.
- 〔11〕LaurendeB ,sylivieD ,MonigueA .TypeⅡtransgtulami naseexpressioninrabbitarticularchondrocytesinculture:relationwithcelldifferentiationcellgrowthcelladhesionandcellapoptosis[J].BiochemBiophysiActa,1996,1312:117124.