张勇;赵建宁;韩宁波;

• 1:第二军医大学南京临床医学院南京军区南京总医院骨科

摘要(Abstract)：

探讨病理状态下软骨细胞能否诱导脂肪干细胞(ADSC)向软骨细胞分化以及可能的最佳条件,以便为临床修复关节软骨损伤提供可能的新途径。[方法]分离共培养新西兰大白兔骨关节炎模型的ADSC和软骨细胞,根据不同血清浓度(10%FBS和2%FBS)和不同培养空间(纤维蛋白凝胶支架和无支架单层培养)分组,共培养14d后,胰酶消化终止。倒置显微镜观察和透射电镜观察共培养后ADSC的形态变化。甲苯胺蓝染色法和免疫细胞化学检测共培养后ADSC的蛋白多糖和Ⅱ型胶原的表达水平。RT-PCR检测蛋白多糖和Ⅱ型胶原的基因转录水平。[结果]共培养7d后部分ADSC变圆,14d时ADSC形态高度分化与成熟软骨细胞相似,其蛋白多糖和Ⅱ型胶原的基因转录和蛋白表达均增高,尤以10%FBS支架共培养组最为明显。[结论]与病理状态下软骨细胞共培养后,ADSC可以被诱导成软骨样细胞。高浓度血清三维培养可以增强这种诱导作用。

关键词(KeyWords)： 脂肪干细胞;软骨细胞;共培养;组织工程

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 张勇;赵建宁;韩宁波;

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参考文献(References)：

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