倪国新;姚志鹏;黄国涛;刘文革;吕维加;

• 1:福建医科大学医学技术与工程学院康复系
• 2:南方医科大学南方医院创伤骨科
• 3:福建医科大学协和医院骨科
• 4:福建省福清市医院骨科

摘要(Abstract)：

[目的]探讨羟基磷灰石(HA)中掺锶对成骨细胞增殖、分化和矿化的影响,以及HA材料中适宜的掺锶量。[方法]用掺锶量分别为1%,5%和10%的HA生物陶瓷粉末及纯HA生物陶瓷粉末制备的浸提液培养SD大鼠成骨细胞,在不同时间点检测成骨细胞增殖、碱性磷酸酶(ALP)活性、核心结合因子(cbfal)基因表达,以及矿化结节形成的情况。[结果]各组成骨细胞的增殖无明显差别(P>0.05);细胞培养第14、21d,掺锶各组成骨细胞的ALP活性、cbfalmRNA表达,及矿化结节数量等方面均显著高于HA组(P<0.01),其中以掺锶量5%组最高,但与10%组相比无显著差异。[结论]HA中掺锶能上调成骨细胞cbfalmRNA的表达,促进成骨细胞分泌碱性磷酸酶,从而促进成骨细胞的分化、矿化,促进骨形成。详细的作用机制及HA中最佳的掺锶量尚需进一步的研究。

关键词(KeyWords)： 锶;羟基磷灰石;成骨细胞;分化;矿化

Abstract:

Keywords:

基金项目(Foundation): 福建省青年人才基金(编号:2007F3040);; 福建省教育厅课题(编号:JA07080)

作者(Author): 倪国新;姚志鹏;黄国涛;刘文革;吕维加;

Email:

DOI:

参考文献(References)：

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