PAI-1 4G/5G基因多态性与严重急性呼吸综合征后骨坏死Relationship between Post-SARS osteonecrosis and PAI-1 4G/5G gene polymorphisms
孙伟;李子荣;史振才;王佰亮;丛笑;韩呈武;
摘要(Abstract)：
[目的]测定严重急性呼吸窘迫综合征(SARS)后骨坏死患者低纤溶倾向的纤溶酶原激活抑制物PAI和相关基因PAI-4G/5G基因多态性与国人SARS后骨坏死的关系,以便于非创伤性骨坏死的早期诊断和易感人群的筛选。[方法]取61例SARS后骨坏死患者抽取空腹肘静脉血。另取健康人群52名为对照,应用凝血仪、酶联免疫吸附(ELISA)方法测定PAI值,应用PCR+固相寡核苷酸连接分析(SPOLA)法测定PAI-1基因4G/5G多态性。[结果]SARS后骨坏死患者PAI异常明显PAI(15·64±13·85)U/mlVS(7·96±4·27)U/ml,PAI-1基因4G/4G在骨坏死人群中增高,但统计学未见差异性,血浆PAI活性与4G/4G纯合子基因型密切相关。[结论]SARS后骨坏死患者的凝血指标异常,PAI可作为骨坏死易感因素的筛选指标。PAI-1基因4G/4G可能是国人骨坏死的遗传标记。
关键词(KeyWords)： 骨坏死;SARS;纤溶酶原激活抑制物
基金项目(Foundation): 首都医学发展基金重大联合项目(2002-1007);; 国家自然科学基金面上项目(NO30672117);; 中日友好医院博士启动课题(NO20056150);; 北京医学会骨科分会默沙东骨质疏松科研项目(2007);; 卫生部属(管)临床重点学科项目(NO2007-2009)
作者(Author): 孙伟;李子荣;史振才;王佰亮;丛笑;韩呈武;
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