林翔;杨军林;苏培强;邹学农;谢超凡;王其飞;黄紫房;

• 1:中山大学附属第一医院脊柱外科

摘要(Abstract)：

[目的]应用基因芯片技术筛选青少年特发性脊柱侧凸(AIS)骨髓间充质干细胞(hBMSCs)成脂分化相关的差异表达基因。[方法]收集AIS及同性别年龄正常对照骨髓标本,密度梯度离心法分离MSCs并培养传代,取第3代MSCs进行成脂诱导2周后,提取各标本的总RNA,反转录成cDNA,并以两种荧光Cy5和Cy3标记后作为探针与人类全基因组表达谱基因芯片杂交,扫描芯片荧光信号图像后用基因图像分析软件进行数字化处理和分析。[结果]筛选出显著表达差异基因1 380条,其中表达上调基因874条,表达下调基因506条。[结论]AIS来源MSCs成脂分化相关差异表达的1 380条基因可能参与了AIS的发生和发展。

关键词(KeyWords)： 青少年特发性脊柱侧凸;骨髓间充质干细胞;成脂分化;基因芯片

Abstract:

Keywords:

基金项目(Foundation): 国家自然基金资助项目(编号:81071439)

作者(Author): 林翔;杨军林;苏培强;邹学农;谢超凡;王其飞;黄紫房;

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DOI:

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