高琪乐;张宏其;郭超峰;唐明星;李艳冰;易小平;刘少华;王昱翔;邓盎;肖力戈;

• 1:中南大学湘雅医院脊柱外科湘雅脊柱外科中心
• 2:中南大学湘雅医院检验科
• 3:中南大学湘雅医院放射科

摘要(Abstract)：

[目的]构建脊柱结核小鼠模型并分析其椎体的骨密度改变。[方法]60只8周龄雌性C57BL/6小鼠随机分成实验组和对照组,每组30只。实验组小鼠脊柱局部注射浓度为108CFU/ml的H37Rv标准菌株悬液0.1 ml,对照组注射生理盐水0.1 ml。饲养12周后取脊柱标本送Micro-CT扫描。获得三维松质骨图像后,评估两组小鼠椎体骨小梁数量及骨小梁厚度差异。[结果]共6只小鼠在Micro-CT上发现了脊柱破坏的病灶。破坏的椎体骨小梁数及骨小梁厚度显著低于未受累的椎体(t=-5.710,P<0.001)和对照组相同节段椎体(t=-6.771,P<0.001),脊柱结核小鼠的椎体平均骨小梁数量较对照明显下降(t=-4.716,P<0.001)。虽然病灶椎的总体骨小梁数量及厚度降低,但在病灶周围存在大量硬化骨形成,病灶局部骨小梁数量及厚度显著增高(t=8.987,P<0.001)。[结论]小鼠脊柱结核病椎平均骨小梁密度下降,但病灶局部骨小梁密度增高。

关键词(KeyWords)： 脊柱结核;小鼠模型;骨小梁;骨密度

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(编号:81401818;81301576)

作者(Author): 高琪乐;张宏其;郭超峰;唐明星;李艳冰;易小平;刘少华;王昱翔;邓盎;肖力戈;

Email:

DOI:

参考文献(References)：

• [1]Zhou Y.Matrix metalloproteinase-1 promoter-1607 bp 1G/2Gpolymorphism associated with increased risk of spinal tuberculosis in Southern Chinese Han population[J/OL].J Clin Lab Anal,Epub ahead of print,2017.DOI:10.1002/jcla,22B6.
• [2]Schirmer P,Renault CA,Holodniy M.Is spinal tuberculosis contagious[J].Int J Infect Dis,2010,14(8):e659-666.
• [3]占玲俊,卢锦标,唐军,等.耐药结核分枝杆菌潜伏-复发感染动物模型的研究进展[J].微生物与感染,2016,11(1):59-64.
• [4]乔永杰,李松凯,甄平,等.不同浓度结核菌建立兔脊柱结核模型的对比研究[J].中国矫形外科杂志,2017,25(5):459-465.
• [5]Acosta A,Norazmi MN,Hernandez-Pando R,et al.The importance of animal models in tuberculosis vaccine development[J].Malays J Med Sci,2011,18(1):5-12.
• [6]Animal models of tuberculosis for vaccine development[J].Ind JMed Res,2009,129(1):11-18.
• [7]梁艳,吴雪琼,李宁,等.结核分枝杆菌感染小鼠模型的建立与评价[J].中国病原生物学杂志,2014(9):775-778.
• [8]王云玲,白山·军斯汗,王红,等.MR-PWI评价早期兔脊柱结核血流灌注的初步研究[J].临床放射学杂志,2013,32(12):1793-1797.
• [9]Kager LM,Runge JH,Nederveen AJ.A new murine model to study musculoskeletal tuberculosis(short communication)[J].Tuberculosis,2014,94(3):306-310.
• [10]Biederer T,Scheiffele P.Mixed-culture assays for analyzing neuronal synapse formation[J].Nat Protoc,2007,2(3):670-676.
• [11]Chen YY,Feng JY,Ting WY,et al.Increased risk of incident osteoporosis and osteoporotic fracture in tuberculosis patients:a population-based study in a tuberculosis-endemic area[J].Osteoporosis Int,2017,28(5):1777-1721.
• [12]Yeh JJ,Wang YC,Lin CC,et al.Association of respiratory tuberculosis with incident bone fracture:bridging the tuberculosis airway infection and the osteoporotic bone[J].Plos One,2016,11(12):e0168673.
• [13]Li X,He L,Hu Y,et al.Sinomenine suppresses osteoclast formation and mycobacterium tuberculosis H37Ra-induced bone loss by modulating RANKL signaling pathways[J].Plos One,2013,8(9):e74274.
• [14]Turnbull ER,Drobniewski F.Vitamin D supplementation:a comprehensive review on supplementation for tuberculosis prophylaxis[J].Expert Rev Respir Med,2015,9(3):269-275.
• [15]Lau RY,Guo X.A review on current osteoporosis research:with special focus on disuse bone loss[J].J Osteoporos,2011,2011:293808.
• [16]Alexandre C,Vico L.Pathophysiology of bone loss in disuse osteoporosis[J].Joint Bone Spine,2011,78(6):572-576.2017-06-01 2017-10-11