小鼠移植性S180肉瘤模型的建立及温和加热对热休克蛋白70表达的影响Establishment of the transplantation model of Sl80 sarcoma and influence to the expression of heat shock protein 70 (HSP 70) induced by mild hyperthermia
汤宇,崔雪梅,袁玫,卢世璧,黄靖香,孙明学
摘要(Abstract)：
目的 :研究NH小鼠S180肉瘤细胞移植后肿瘤模型生长特点 ,以及热休克蛋白 (heatshockproteinsHSPs)70在温和加热 (4 2℃ )不同时段表达变化。方法 :不同剂量S180肉瘤细胞于NH小鼠背部皮下接种 ,观察肿瘤组织生长规律 ,取部分肉瘤细胞进行热休克处理 2、 4、 6、 8、 10、 12h后 ,进行HSP 70免疫组化观察 ,结果 :肿瘤发生率10 0 % ,不同剂量组的肿瘤生存期限差异有显著意义 ,肿瘤生长大小无显著差异 ;对HSPs的免疫组化观察表明 :热休克处理后HSP 70阳性增强 ,以 6h后最明显 ;8h后细胞发生皱缩、溶解。结论 :此模型是HSPs/肽复合物提取、纯化中稳定的动物肿瘤疫苗治疗模型 ;分离、纯化HSP 70时将肿瘤组织热休克处理 (4 2℃ ) 6h即可。
关键词(KeyWords)： 肿瘤模型;热休克蛋白70;免疫组化
基金项目(Foundation): 总后专项科研基金资助
作者(Author): 汤宇,崔雪梅,袁玫,卢世璧,黄靖香,孙明学
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