兔脂肪干细胞的分离培养鉴定及成骨诱导分化研究Osteogenic differentiation of adipose-derived stem cells from rabbits in vitro
郝伟;胡蕴玉;魏义勇;庞龙;白建萍;吕荣;王军;张大伟;
摘要(Abstract)：
[目的]探讨在成骨诱导条件下兔脂肪干细胞的体外诱导分化情况。[方法]取3个月龄日本大耳白兔颈背部皮下脂肪,用Ⅰ型胶原酶消化获得细胞。Stro-1免疫细胞化学染色鉴定细胞性质;加入成骨诱导液后依次进行形态学、Ⅰ型胶原、碱性磷酸酶及钙盐沉积相关检测。[结果](1)原代所获细胞Stro-1表达阳性。(2)在诱导条件下,Ⅰ型胶原、碱性磷酸酶及钙盐沉积均呈阳性表达。[结论]脂肪干细胞来源广、易获取、对机体创伤小,与骨髓间充质干细胞类似,经体外诱导后可实现成骨分化,为骨组织工程提供了一种新的种子细胞。
关键词(KeyWords)： 兔;脂肪干细胞;成骨分化;骨组织工程
基金项目(Foundation): 全军医药卫生科研基金课题(No.100211)
作者(Author): 郝伟;胡蕴玉;魏义勇;庞龙;白建萍;吕荣;王军;张大伟;
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