兔脂肪干细胞的分离培养鉴定及成骨诱导分化研究Osteogenic differentiation of adipose-derived stem cells from rabbits in vitro
郝伟;胡蕴玉;魏义勇;庞龙;白建萍;吕荣;王军;张大伟;
摘要(Abstract):
[目的]探讨在成骨诱导条件下兔脂肪干细胞的体外诱导分化情况。[方法]取3个月龄日本大耳白兔颈背部皮下脂肪,用Ⅰ型胶原酶消化获得细胞。Stro-1免疫细胞化学染色鉴定细胞性质;加入成骨诱导液后依次进行形态学、Ⅰ型胶原、碱性磷酸酶及钙盐沉积相关检测。[结果](1)原代所获细胞Stro-1表达阳性。(2)在诱导条件下,Ⅰ型胶原、碱性磷酸酶及钙盐沉积均呈阳性表达。[结论]脂肪干细胞来源广、易获取、对机体创伤小,与骨髓间充质干细胞类似,经体外诱导后可实现成骨分化,为骨组织工程提供了一种新的种子细胞。
关键词(KeyWords): 兔;脂肪干细胞;成骨分化;骨组织工程
基金项目(Foundation): 全军医药卫生科研基金课题(No.100211)
作者(Author): 郝伟;胡蕴玉;魏义勇;庞龙;白建萍;吕荣;王军;张大伟;
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参考文献(References):
- (1)王少山,李宝锋,王楠,等.骨髓基质干细胞体外培养及临床应用研究[J].中国矫形外科杂志,2004,12(4):1086-1088.
- (2)Auqu ier P,Macquart-Mou lin D,Moatti JP,et al.Comparison of anxie-ty,pain and d iscom fort in two procedures of hematopoiefic stem cellcollection:leukacytapheresis and bone marrow harvest[J].Bone Mar-row Transplant,1995,16(4):541-547.
- (3)Zok PA,Zhu M,M izuno H,et al.Mu ltilineage cells from human ad i-pose tissue:implications for cellbased therap ies[J].Tissue Eng,2001,7:211-228.
- (4)D e Ugarte DA,Morizono K,E lbarbary A,et al.Comparison of mu lti-lineage cells from human ad ipose tissue and bone marrow[J].CellsTissues O rgans,2003,174(3):101-109.
- (5)D e Ugarte DA,A lfonso Z,Zuk PA,et al.D ifferential expression of stemcellmob ilization-associated molecu les on mu lti-lineage cells from ad i-pose tissue and bone marrow[J].Immunol Lett,2003,89(2~3):267-270.
- (6)Zuk PA,Zhu M,Ash jian P,et al.Human ad ipose tissue is a source ofmu ltipotent stem cells[J].Mol B iol Cell,2002,13(12):4279-4295.
- (7)Batin ic D,Marusic M,Pavletic Z,et al.Relationsh ip between d ifferingvolum es of bone marrow asp irates and their cellu lar composition[J].Bone Marrow Transplant,1990,6(2):103-107.
- (8)G imb le JM,Gu ilak F.Ad ipose-derived adu lt stem cells:isolation,char-acterization,and d ifferentiation potential[J].Cytotherapy,2003,5(5):362-369.
- (9)SeoM J,Suh SY,Bae YC,et al.D ifferentiation of human ad ipose stro-mal cells into hepatic lineage in vitro and in vivo[J].B iochem B io-phys Res Commun,2005,32(1):258-264.
- (10)Owen TA,Aronow MA,Shalhoub V,et al.Progressive developm ent ofthe rat osteob lasl phenotype in vitro:reciprocal relationsh ips in the ex-pression of genes associated w ith osteob last proliferation and d ifferenti-ation daring formation of the bone extracellu larmatrix[J].J Cell Phys-iol,1990,143:420-430.
- (11)P ittengerMF,Mackay AM,Beck SC,et al.Mu ltilineage potential of a-du lt human m esenchymal stem cells[J].Science,1999,284(5411):143-1477.
- (12)Denn is JE,Carb illet JP,Caplan AL,et al.The Stro-1+marrow cell pop-ulation is multipotential[J].Cells Tissues Organs,2002,170:73-82.
- (13)Jason R,B rian C,David S,et al.Characterization of growth and osteo-gen ic d ifferentiation of rabb it bone marrow stromal cells[J].J SurgRes,2006,article in press.
- (14)李建军,傅永慧,孙鸿斌,等.骨形态发生蛋白2基因治疗与缓释载体修复骨缺损的比较研究[J].中国矫形外科杂志,2005,13(17):1334-1336.