种子细胞-支架复合体在骨组织修复中的研究进展
李吉鹏;邓国英;赵庆华;
摘要(Abstract):
<正>针对人体由于创伤、感染、肿瘤、先天畸形等各种原因引起的骨缺损,传统治疗方法多采取自体骨、异体骨移植等手段。虽然自体骨移植作为骨缺损修复的"金标准",但是自体骨取材具有局限性,而异体骨存在的排斥反应、感染等弊端,均成为骨组织修复中无法回避的难题。因此,研究一种理想的人骨代替物已成为骨组织工程的重大课题。良好的人骨替代物通常要符合以下标准:(1)具有良好的生物相容性,降解产物
关键词(KeyWords): 骨组织工程;支架材料;种子细胞
基金项目(Foundation): 上海交通大学“医工(理)交叉研究基金”项目(编号:YG2011MS28)
作者(Author): 李吉鹏;邓国英;赵庆华;
Email:
DOI:
参考文献(References):
- [1]Burg KJL,Porter S,Kellan JF.Biomaterial developments for bone tissueengineering[J].Biomaterials,2000,23:2347-2359.
- [2]Rezwana K,Chen QZ,Blakera JJ,et al.Biodegradable and bioactiveporous polymer/inorganic composite scaffolds for bone tissue engineer-ing[J].Biomaterials,2006,18:3413-3431.
- [3]Tuan VA,Li S,Falconer JL,et al.Separating organics from water bypervaporation with isomorphously-substituted MFI zeolite membranes[J].J Membr Sci,2002,196:111-123.
- [4]Nilufer D,Hilmioglu,ST.Pervaporation of MTBE/methanol mixturesthrough PVA membranes[J].Desalination,2004,160:263-270.
- [5]Frazier DD,Lathi VK,Gerhart TN,et al.Ex vivo degradation of a poly(propylene gly col-fumarate)biodegradable particulate composite bonecement[J].J Biomed Master Res,1997,3:383-389.
- [6]Kharas GB,Kamenetsky M,Simantirakis J,et al.Synthesis and charac-terization of fumarate-based polyesters for use in bioresorbable bone ce-ment composites[J].J Appl Polym Sci,1997,6:1123-1137.
- [7]del Real RP,Wolke JG,Vallet-RegiM,et al.A new method to producemacropores in calcium phosphate cements[J].Biomaterials,2002,17:3673-3680.
- [8]Yang XC,Chen XN,Wang HJ,et al.Acceleration of osteogenic differ-entiation of preosteoblastic cells by chitosan containing nanofibrousscaffolds[J].Biomacromolecules,2009,10:2772-2778.
- [9]岳进,郭大刚,孙翔,等.掺锶磷酸钙骨水泥的体外细胞生物学性能评价及其与掺锶量的相关性[J].西安交通大学学报医学版,2008,5:522-526.
- [10]邵英,张舵,荣莉,等.聚L-乳酸/聚L-乳酸接枝纳米羟基磷灰石复合材料对成骨细胞黏附和增殖能力的影响[J].中国组织工程研究与临床康复,2009,34:6617-6621.
- [11]Malheiro VN,Spear RL.Osteoblast and monocyte responses to 444 fer-ritic stainless steel intended for a magneto-mechanically actuated fi-brous scaffold[J].Biomaterials,2011,29:6883-6892.
- [12]Chan J,O'Donoghue K,de la Fuente J,et al.Human fetal mesenchymalstem cells as vehicles for gene delivery[J].Stem Cells,2005,1:93-102.
- [13]段智霞,郑启新,郭晓东.BMSCs在PLGA-[ASP-PEG]基质材料表面粘附及增殖的研究[J].中国生物工程杂志,2006,12:86-91.
- [14]Zhang H,Wang W,Chu D,et al.Preliminary study on chitosan/HAPbilayered scaffold[J].Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi,2008,11:1358-1363.
- [15]Huan SW,Zha ZG,Yao P,et al.Propylene carbonate/chitosan nanofi-ber porous scaffolds composited with bone marrow mesenchymal stemcell for bone defect repair in rabbits[J].Zhongguo Zuzhi GongchengYanjiu yu Linchuang Kangfu,2011,16:2889-2894.
- [16]Mu XH,Zhao ZY,Xu L,et al.Nano-bone composite of bone marrowstromal stem cells and nano-material scaffold in repairing rabbit femoralhead necrosis[J].Zhongguo Zuzhi Gongcheng Yanjiu yu LinchuangKangfu,2010,51:9582-9586.
- [17]Huang YX,Ren J,Ren TB,et al.Bone marrow stromal cells cultured onpoly(lactide-co-glycolide)/nano-hydroxyapatite composites with chem-ical immobilization of Arg-Gly-Asp peptide and preliminary bone regen-eration of mandibular defect thereof[J].J Biomed Mater Res A,2010,4:993-1003.
- [18]Teixeira S,Ferraz MP,Ferraz MP,et al.Proliferation and mineralizationof bone marrow cells cultured on macroporous hydroxyapatite scaffoldsfunctionalized with collagen type I for bone tissue regeneration[J].JBiomed Mater Res A,2010,1:1-8.
- [19]Jiang XQ,Zhao J,Wang S,et al.Mandibular repair in rats with premi-neralized silk scaffolds and BMP-2-modified bMSCs[J].Biomaterials,2009,27:4522-4532.
- [20]Bhardwaj N,Kundu SC.Chondrogenic differentiation of rat MSCs onporous scaffolds of silk fibroin/chitosan blends[J].Biomaterials,2012,10:2848-2857.
- [21]Hasegawa T,Miwa M,Sakai Y,et al.Efficient cell-seeding into scaf-folds improves bone formation[J].J Dent Res,2010,8:854-859.
- [22]Ben-David D,Kizhner T,Livne E,et al.A tissue-like construct of hu-man bone marrow MSCs composite scaffold support in vivo ectopic boneformation[J].J Tissue Eng Regen Med,2010,1:30-37.
- [23]Cui G,Li J,Lei W,et al.The mechanical and biological properties of aninjectable calcium phosphate cement-fibrin glue composite for bone re-generation[J].J Biomed Mater Res B Appl Biomater,2010,2:377-385.
- [24]Weir MD,Xu HH.Human bone marrow stem cell-encapsulating calciumphosphate scaffolds for bone repair[J].Acta Biomater,2010,10:4118-4126.
- [25]Mueller SM,Glowacki J.Age-related decline in the osteogenic potentialof human bone marrow cells cultured in three-dimensional collagensponges[J].J Cellular Biochem,2001,4:583-590.
- [26]Zhao L,Burguera EF,Xu HH,et al.Fatigue and human umbilical cordstem cell seeding characteristics of calcium phosphate-chitosan-biode-gradable fiber scaffolds[J].Biomaterials,2010,5:840-847.
- [27]Xu HH,Zhao L,Weir MD.Stem cell-calcium phosphate constructs forbone engineering[J].J Dent Res,2010,12:1482-1488.
- [28]Tang M,Weir MD,Xu HH.Mannitol-containing macroporous calciumphosphate cement encapsulating human umbilical cord stem cells[J].JTissue Eng Regen Med,2012,3:214-224.
- [29]Zhao L,Weir MD,Xu HH.An injectable calcium phosphate-alginatehydrogel-umbilical cord mesenchymal stem cell paste for bone tissueengineering[J].Biomaterials,2010,25:6502-6510.
- [30]Bao C,Chen W,Weir MD.Effects of electrospun submicron fibers incalcium phosphate cement scaffold on mechanical properties and osteo-genic differentiation of umbilical cord stem cells[J].Acta Biomater,2011,11:4037-4044.
- [31]隋杰,李志忠,孙国栋,等.聚左旋乳酸多孔支架材料复合人脐带间充质干细胞的异位成骨[J].中国组织工程研究与临床康复,2011,12:2120-2124.
- [32]Gauthaman K,Venugopal JR,Yee FC,et al.Osteogenic differentiationof human Wharton's jelly stem cells on nanofibrous substrates in vitro[J].Tissue Eng Part A,2011,1-2:71-81.
- [33]Jager M,Krauspe R.Antigen expression of cord blood derived stem cellsunder osteogenic stimulation in vitro[J].Cell Biol Int,2007,9:950-957.
- [34]Jager M,Degistirici O,Knipper A,et al.Bone healing and migration ofcord blood-derived stem cells into a critical size femoral defect after xe-notransplantation[J].J Bone Miner Res,2007,8:1224-1233.
- [35]Liu G,Li Y,Sun J.In vitro and in vivo evaluation of osteogenesis of hu-man umbilical cord blood-derived mesenchymal stem cells on partiallydemineralized bone matrix[J].Tissue Eng Part A,2010,3:971-982.
- [36]丁然,李锋.人转化生长因子β3基因转染KLD-12自组装纳米肽纤维支架三维培养前软骨干细胞[J].中国组织工程研究与临床康复,2010,29:5339-5343.