可控微结构EBM钛合金支架与成骨细胞的体外三维复合培养Three-dimensional culture of osteoblast seeded on titanium alloy scaffold with controlled internal structure fabricated using electron beam melting techniques in vitro
李国臣;王林;桑宏勋;李祥;王成焘;黄鑫;雷伟;姥伟;
摘要(Abstract)：
[目的]探讨可控微结构EBM钛合金支架作为成骨细胞体外培养载体的可行性,并观察载体类蜂巢状孔结构在成骨细胞培养中的作用。[方法]应用直接金属快速成形技术—电子束熔化成形(electron beam melting,EBM)过程直接构建和制备可控性微结构钛合金支架。分离培养1d龄胎兔颅骨成骨细胞,以兔颅骨源性成骨细胞复合支架作为实验组,以单独培养的兔颅骨源性成骨细胞作为对照组。将成骨细胞与支架复合培养1、7、14d后,进行倒置相差显微镜、扫描电镜及组织切片染色观察细胞与材料复合情况,以MTT比色法及碱性磷酸酶的定量检测研究材料结构对细胞增殖、分化的影响。[结果]成骨细胞/支架复合培养7d后,大量细胞通过伸出多个伪足粘附在支架表面以及孔周围并与支架牢固结合;培养14d后支架表面及孔隙内有大量细胞分布,细胞伸出数个突起沿着孔隙壁逐渐向孔隙内延伸、汇集。支架上的细胞增殖、分化以及转移明显增加,与对照组比较有明显统计学差异(P<0.05)。[结论]EBM钛合金支架有利于细胞的贴附、生长及增殖,并对细胞的功能无不良的影响。支架类蜂巢状孔结构能够调节成骨细胞在支架内的分布。
关键词(KeyWords)： 组织工程;支架;钛合金;多孔结构
基金项目(Foundation): 陕西省科学技术研究发展计划资助项目(2008K14-01)
作者(Author): 李国臣;王林;桑宏勋;李祥;王成焘;黄鑫;雷伟;姥伟;
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