明胶/纳米羟基磷灰石三维多孔骨支架的制备及其生物安全性评价Study on preparation of gelatin/nanohydroxypatite scaffold with three-dimension porous structure and evaluation of its biocompatibility
刘越;赵艳梅;田学忠;崔赓;杨强;夏群;张伯勋;
摘要(Abstract)：
[目的]探讨冷冻干燥法制备明胶/纳米羟基磷灰石(nano-hydroxyapatite,nHA)三维多孔支架的可行性并评价材料的生物安全性。[方法]取明胶水溶液,将其分别与10 wt%、20 wt%、30 wt%的nHA混合,交联后采用冷冻干燥法制备明胶/nHA三维多孔支架。评价材料的理化性质,通过测定支架孔径、孔隙率、吸水率、抗压强度及降解pH值优化制备条件;MTT法分析支架浸提液毒性;共培养观察细胞在材料表面及周围生长情况;材料埋入背部肌肉内,组织学染色观察评价其生物安全性。[结果]体视显微镜及电镜显示,制备的支架均呈三维多孔隙结构。随着nHA含量的增加,材料成孔效果变差,孔径、孔隙率、吸水率变小,而抗压强度增加。其中以nHA含量为20 wt%的材料表现更为适宜,并选其进行生物相容性实验。MTT法显示不同浓度支架浸提液与对照DMEM培养液吸光度值比较,差异无统计学意义(P>0.05)。细胞与材料共培养生长状态良好。HE染色观察材料与周围肌肉组织无排斥反应,组织相容性良好。[结论]制备的明胶/nHA支架材料具有三维孔隙结构,具备合适的孔径和孔隙率,无毒,生物相容性良好,可作为骨组织工程支架使用。
关键词(KeyWords)： 纳米羟基磷灰石;明胶;支架;组织工程
基金项目(Foundation): 国家自然基金资助项目(编号31000432);; 天津市卫生局科技基金(编号:2012KY24)
作者(Author): 刘越;赵艳梅;田学忠;崔赓;杨强;夏群;张伯勋;
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