贺斌;陶海鹰;刘世清;卫爱林;胡明慧;

• 1:武汉大学人民医院骨科
• 2:武汉大学中南医院骨科

摘要(Abstract)：

[目的]探讨羧甲基壳聚糖(carboxymethylated chitosan,CMCS)对氧化应激诱导雪旺细胞(schwann cells,SCs)凋亡的保护作用及作用机制。[方法]体外培养SCs,S-100免疫荧光染色鉴定。将SCs分为空白对照组、H2O2诱导组、H2O2加CMCS处理组。通过CCK-8检测不同组SCs增殖情况,流式细胞仪计数检测SCs早期凋亡率,检测各组细胞内超氧化物歧化酶(SOD)及丙二醛(MDA)的含量,Real-time PCR技术检测Bcl-2及Bax mRNA表达水平,Western blot法检测Bcl-2,Bax,Caspase-3、-9的表达水平。[结果]H2O2诱导SCs后其细胞增殖活性明显降低,加入不同浓度CMCS后增殖活性增加。流式细胞技术检测结果表明,经H2O2诱导SCs早期凋亡率增加,加入50~200μg/ml CMCS后凋亡率逐渐降低。经H2O2诱导组SCs与空白对照组比较SOD含量明显减少,MDA含量明显增加(P<0.05);加入CMCS后SOD含量增加,MDA含量减少(P<0.05)。Real-time PCR及Western blot检测结果表明经H2O2诱导SCs内Bcl-2表达降低,Bax,Caspase-3、-9表达增高,加入CMCS后Bcl-2表达恢复,Bax,Caspase-3、9表达抑制。[结论]CMCS对H2O2诱导SCs凋亡具有保护作用。

关键词(KeyWords)： 羧甲基壳聚糖;雪旺细胞;细胞凋亡;氧化应激

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(编号:81301056,30600627);; 湖北省自然科学基金资助项目(编号:2013CKB002)

作者(Author): 贺斌;陶海鹰;刘世清;卫爱林;胡明慧;

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DOI:

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