细胞凋亡在失神经骨折愈合过程中的作用机制The role of apoptosis during the process of fracture healing in a denervation state
吴卓;赵敏;王永红;汪玉良;汪静;马靖琳;张波;刘松;
摘要(Abstract)：
[目的]通过观察失神经后大鼠胫骨骨折愈合情况,探讨细胞凋亡在失神经骨折愈合过程中发生的规律及意义。[方法]将96只成年健康雌性SD大鼠随机分为实验组和对照组。实验组建立T10脊髓完全性损伤状态下大鼠胫骨骨折模型,对照组行单纯胫骨骨折术,分别于术后3、5、7、14、21及28 d取材(每个时间点n=8),于骨折断端上下各5 mm处剪断胫骨,经脱钙及固定后,制成6μm切片。切片应用TUNEL法原位观察细胞凋亡的时间分布。另外还包括动物行为学观察以及术后7、14、21和28 d拍摄X线片。[结果]在骨折愈合过程中,两组均可见凋亡细胞,实验组的凋亡指数在术后各时间点均高于对照组,差异具有统计学意义(P<0.05),并具有时间分布的特点,两组均以术后7 d出现高峰,随着时间的延长,实验组和对照组的细胞凋亡指数逐渐减小。[结论]细胞凋亡是脊髓损伤后重要的病理变化,机体通过凋亡机制控制细胞数量并清除无用的细胞,以利于失神经状态下的骨折修复愈合。
关键词(KeyWords)： 细胞凋亡;失神经;骨折愈合;脊髓损伤;TUNEL法
基金项目(Foundation): 甘肃省科技支撑计划(编号:1304FKCA091)
作者(Author): 吴卓;赵敏;王永红;汪玉良;汪静;马靖琳;张波;刘松;
Email:
DOI:
参考文献(References)：
- [1]Li J,Liu G,Zheng Y,et al.The epidemiological survey of acute traumatic spinal cord injury(ATSCI)of 2002 in Beijing municipality[J].Spinal Cord,2011,7:777-782.
- [2]邵为,花曼曼,龚凯,等.EGb761对急性大鼠脊髓损伤后血脊髓屏障的保护作用及其机制研究[J].中国矫形外科杂志,2011,2:131-135.
- [3]Chen C,Chen Q,Mao Y,et al.Hydrogen-rich saline protects against spinal cord injury in rats[J].Neurochem Res,2010,7:1111-1118.
- [4]Liu J,Tang T,Yang H.Protective effect of deferoxamine on experimental spinal cord injury in rat[J].Injury,2011,8:742-745.
- [5]Chen HC,Fong TH,Lee AW,et al.Autophagy is activated in injuredneurons and inhibited by methylprednisolone after experimental spinal cord injury[J].Spine(Phila Pa 1976),2012,6:470-475.
- [6]崔开,韩亚新,孔冉冉,等.大鼠急性脊髓损伤后Caspase-3、Cathep sin B的表达[J].中国矫形外科杂志,2009,18:1400-1403.
- [7]Chen Y,Klionsky DJ.The regulation of autophagy unanswered questions[J].J Cel Sci,2011,2:161-170.
- [8]Marzetti E,Hwang JC,Lees HA,et al.Mitochondrial death effectors:relevance to sarcopenia and disuse muscle atrophy[J].Biochim Biophys Acta,2010,3:235-244.
- [9]Garzón-Alvarado DA,Ramírez-Martínez AM,Cardozo de Martínez CA.Numerical test concerning bone mass apposition under electrical and mechanical stimulus[J].Theor Biol Med Model,2012,14:1-17.
- [10]Sabarinathan D,Mahalakshmi P,Vanisree AJ.Naringenin promote apoptosis in cerebrally implanted C6 glioma cells[J].Mol Cell Biochem,2010,1-2:215-222.
- [11]Zamli Z,Sharif M.Chondrocyte apoptosis:a cause or consequence of osteoarthritis[J].Int J Rheum Dis,2011,2:159-166.