腺病毒介导的NT-3基因在骨髓间质干细胞中的表达Expression of adenoviral vector-mediated NT-3 gene transfer into rat mesenchymal stem cells in vitro
彭松林,方煌,黎逢峰,李锋,陈安民
摘要(Abstract)：
目的 :研究腺病毒介导的NT 3基因在培养的大鼠骨髓间质干细胞 (mesenchymalstemcells ,MSCs)中的表达。方法 :在 2 93细胞中培养扩增NT 3重组腺病毒 (adenovirusvectorforNT 3 ,Ad NT 3 ) ,测定病毒滴度 ,然后用Ad NT 3感染传代培养的MSCs ,RT PCR技术检测NT 3基因的表达。结果 :Ad NT 3扩增后获得了较高滴度的病毒 ,MSCs经Ad NT 3感染后有NT 3mRNA的转录。结论 :腺病毒介导的NT 3基因可转入培养的MSCs并高效表达 ,为NT 3基因治疗的研究奠定了基础。
关键词(KeyWords)： 神经营养素3;骨髓间质干细胞;逆转录多聚酶链反应;基因转移
基金项目(Foundation):
作者(Author): 彭松林,方煌,黎逢峰,李锋,陈安民
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参考文献(References)：
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