胡伟华;陈安民;郭风劲;李锋;黄晖;张伟凯;

• 1:华中科技大学同济医学院附属同济医院骨科

摘要(Abstract)：

[目的]从永生化骨骺干细胞中克隆Sox9基因并构建真核表达载体,并探讨Sox9诱导骨髓基质细胞向骨骺干细胞分化的可能性。[方法]以RT-PCR方法获得Sox9全长,插入pGEM-TEasy克隆载体中,测序正确后与pEG-FP-IRES2表达载体酶切后连接,复合质粒以脂质体法转染骨髓基质细胞,观察转染效率,Sox9和FGFR-3的表达。流式细胞术鉴定细胞表型,MTT法检测细胞增殖活性。[结果]成功的完成了Sox9的扩增和表达载体的构建,重组载体转染骨髓基质细胞后能检测到Sox9、FGFR-3的表达,增殖活性与骨骺干细胞无异。[结论]成功构建了Sox9真核表达载体,其能诱导骨髓基质细胞分化为骨骺干细胞并具有其特性。

关键词(KeyWords)： 骨骺干细胞;Sox9;骨髓基质细胞;转染

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(编号:30650006);; 教育部博士点基金资助项目[No.教技发中心函(2005)216]

作者(Author): 胡伟华;陈安民;郭风劲;李锋;黄晖;张伟凯;

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DOI:

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