李强;张柳;李震;穆树林;程潭;田发明;

• 1:华北煤炭医学院附属医院骨科

摘要(Abstract)：

[目的]观察阿仑膦酸钠(alendronate,ALN)对前交叉韧带切断术(anterior cruciate ligament transac-tion,ACLT)后骨关节炎模型大鼠膝关节软骨和软骨下骨的影响。[方法] 24只3个月龄雌性SD大鼠按体重随机分为假手术组(Sham,n=8)、手术组(ACLT+NS,n=8)及给药组(ACLT+ALN,n=8),术后ALN组给予阿仑膦酸钠20μg.kg-1.2 d-1皮下注射,连续给药12周;ACLT组则给予等剂量无菌生理盐水。股骨远端软骨下骨骨密度和胫骨近端骨组织形态计量学分析,通过关节软骨Mankin评分和MMP-13免疫组化染色观察关节软骨的退变和软骨基质的降解程度。[结果] (1)ACLT+NS组股骨内侧髁骨密度显著低于Sham组和ACLT+ALN组(P<0.05);(2)ACLT+ALN组的胫骨近端骨量显著高于ACLT+NS组(P<0.05);(3)ACLT+ALN组的关节软骨Mankin评分和MMP-13免疫组化染色平均积分光密度显著低于ACLT+NS组(P<0.05)。[结论]阿仑膦酸钠20μg.kg-1.2 d-1皮下注射12周可以预防ACLT大鼠关节软骨退变,其作用机制可能与下调MMP-13的表达和增加软骨下骨骨量有关。

关键词(KeyWords)： 骨关节炎;阿仑膦酸钠;软骨下骨;骨组织形态计量学;MMP-13

Abstract:

Keywords:

基金项目(Foundation): 河北省科技领军人才创新基金项目(编号:06547008D-8)

作者(Author): 李强;张柳;李震;穆树林;程潭;田发明;

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DOI:

参考文献(References)：

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