大鼠骨髓间充质干细胞体外诱导失巢凋亡过程中半胱氨酸蛋白酶-3的作用Significance of casepase-3 in induced anoikis of mesenchymal stem cells(MSCs)in vitro
冯建军;杨述华;孙立;唐欣;
摘要(Abstract)：
[目的]探讨在体外诱导骨髓间充质干细胞失巢凋亡过程中半胱氨酸蛋白酶-3(Caspase-3)的作用。[方法]将体外培养的骨髓间充质干细胞随机分为3组:对照组、诱导凋亡组、抑制凋亡组。应用Caspase-3活性检测试剂盒,荧光比色法和W estern b lotting法检测失巢凋亡中Caspase-3活性的改变;借助流式细胞仪分析骨髓间充质干细胞凋亡的变化。[结果]流式细胞术显示诱导凋亡组出现了明显的凋亡峰,凋亡率随诱导时间的延长而明显增加;其它两组的凋亡率较低且较稳定。W estern b lotting检测和Caspase-3活性荧光检测显示,诱导凋亡组Caspase-3的活性以及蛋白表达水平随诱导凋亡时间的延长而明显升高,且与细胞凋亡率相关,以后者更灵敏,其余两组活性无明显变化。[结论]Caspase-3蛋白在诱导骨髓间充质干细胞失巢凋亡的过程中发挥着重要作用;抑制Caspase-3的活性可以有效降低细胞失巢凋亡率。
关键词(KeyWords)： 骨髓间充质干细胞;Caspase-3;失巢凋亡
基金项目(Foundation): 国家自然科学基金资助课题(No.30471753)
作者(Author): 冯建军;杨述华;孙立;唐欣;
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DOI:
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