贺斌;陶海鹰;卫爱林;李孝海;陈任;潘峰;郑啸;

• 1:武汉大学人民医院骨科
• 2:武汉市新洲区人民医院骨科

摘要(Abstract)：

[目的]探讨线粒体途径在羧甲基壳聚糖(carboxymethylated chitosan,CMCS)保护过氧化氢(H_2O_2)诱导雪旺细胞(Schwann cells,SCs)凋亡中的作用及机制。[方法]体外培养SCs,S-100免疫荧光染色鉴定。将SCs分为空白对照组、H_2O_2诱导组、H_2O_2加CMCS处理组。流式细胞仪检测细胞凋亡比率,罗丹明(Rhodamine123)荧光染色检测细胞线粒体膜电位水平,Western blot检测SCs内细胞色素C(Cytochrome C)表达水平。[结果]本实验培养细胞经S-100荧光染色鉴定阳性率达95%以上,H_2O_2诱导SCs凋亡,降低线粒体膜电位,增加细胞色素C释放;而在加入CMCS后SCs凋亡比例降低,线粒体膜电位增加,细胞色素C释放减少。[结论]CMCS通过抑制线粒体细胞凋亡途径保护H_2O_2诱导SCs凋亡。

关键词(KeyWords)： 羧甲基壳聚糖;雪旺细胞;凋亡;线粒体;氧化应激

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(编号:81301056);; 湖北省自然科学基金资助项目(编号:2013CKB002;2015CKB753)

作者(Author): 贺斌;陶海鹰;卫爱林;李孝海;陈任;潘峰;郑啸;

Email:

DOI:

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