壳聚糖纤维及明胶联合增强的磷配钙骨水泥理化性能及生物相容性研究Physiochemical and biocompatible properties of a calcium phosphate cement combined with Chitosan fiber and gelatin
潘朝晖;范清宇;蒋萍萍;王剑利;
摘要(Abstract):
[目的]探讨壳聚糖纤维联合明胶对磷酸钙骨水泥理化性能及生物相容性的影响。[方法]将不同比例的壳聚糖纤维、明胶和磷酸钙骨水泥复合,通过测定复合物抗弯曲强度、分析固化体相组成、观察超微结构及抽提液体外细胞反应,了解壳聚糖纤维-明胶-磷酸钙骨水泥复合材料的理化性能及细胞毒性。[结果]壳聚糖纤维体积比对抗弯强度影响显著(P<0.05),体积比增加到30%,强度为(9.04±2.63)MPa(对照组为(2.48±0.62)MPa,P<0.05)。X线衍射观察各复合物在31°和35°之间均可见与标准羟基磷灰石一致的衍射峰,与对照组相比较,明胶组、10%纤维组和30%纤维组羟基磷灰石特征衍射峰强度相对高,羟基磷灰石相比例增加,磷酸四钙相比例减少。扫描电镜显示各实验组微孔数少于对照组,纤维分散较好,并被水泥湿化。诱导后的大鼠骨髓基质细胞可在复合物表面生长,短期抽提实验证实实验组和阴性对照组细胞增殖显著高于阳性对照(P<0.05),实验组和阴性对照组间无统计学差异。[结论]添加壳聚糖纤维和明胶在一定程度上提高了骨水泥强度,加速骨水泥向羟基磷灰石转化,新型复合材料无细胞毒性。
关键词(KeyWords): 磷酸钙骨水泥;壳聚糖;明胶;纤维;骨髓基质细胞
基金项目(Foundation):
作者(Author): 潘朝晖;范清宇;蒋萍萍;王剑利;
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DOI:
参考文献(References):
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