刘瑞龙;薛少青;纪学尚;周传利;王德春;

• 1:青岛大学医学院附属医院脊柱外科
• 2:山东省胶南市中医医院外二科

摘要(Abstract)：

[目的]观察高渗透压对体外培养的兔髓核细胞凋亡的影响及细胞外信号调节激酶(ERK1/2)信号转导通路在此过程中的作用。[方法]用不同的渗透压梯度以及ERK1/2特异性抑制剂PD 98 059(50μmol/L)分不同作用时间处理髓核细胞;流式细胞仪检测髓核细胞在不同处理组的凋亡情况,蛋白质免疫印迹技术检测各组ERK1/2和p-ERK1/2蛋白的表达情况;免疫荧光标记技术检测p-ERK1/2蛋白在髓核细胞内的分布情况。[结果]高渗透压(600 mOsm)培养基中兔髓核细胞凋亡显著增加,与对照组相比差异有统计学意义(P=0.013),并呈时间依赖性;PD98059预处理组与相应时间段同渗透压组相比,细胞凋亡进一步显著地增加(P=0.035);400 mOsm组及其抑制组在各时间段细胞凋亡均不明显;600 mOsm高渗透压显著激活p-ERK1/2的表达,并且在3 h组表达水平最高,与对照组比较差异显著(P=0.027),而PD98059几乎阻断p-ERK1/2的表达;免疫荧光标记检测到p-ERK1/2在600 mOsm高渗组的细胞胞浆和胞核中均有分布。[结论]高渗透压(600 mOsm)应激下兔髓核细胞凋亡显著增加,而且激活的ERK1/2信号转导通路具有抵抗髓核细胞凋亡的作用;并且髓核细胞能够适应轻度增加的渗透压(400mOsm)环境。

关键词(KeyWords)： ERK1/2/MAPK;高渗透压;细胞凋亡

Abstract:

Keywords:

基金项目(Foundation): 国家自然基金委课题项目(组织工程联合基因治疗修复椎间盘退变的实验研究编号:30672132)

作者(Author): 刘瑞龙;薛少青;纪学尚;周传利;王德春;

Email:

DOI:

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