王泽民;段莉;梁宇杰;王大平;

• 1:广州医科大学
• 2:广东深圳市第二人民医院
• 3:北京大学深圳研究生院

摘要(Abstract)：

[目的]比较体外静态与动态培养两种方式对培养人关节软骨细胞复合Ⅰ型胶原支架材料的效果,从而探索体外构建移植物用于治疗关节软骨缺损的适宜条件及方式。[方法]分离培养人关节软骨细胞,P2代软骨细胞接种于Ⅰ型胶原支架,随机分为3组,A组:静态培养;B组:动态培养;C组:单层培养。采用倒置相差显微镜、荧光显微镜、SEM、HE染色观察细胞在支架上的分布、黏附、生长及形态特点,实时荧光定量PCR检测软骨细胞表型特异基因Ⅱ型胶原的表达情况。[结果]体外单层培养的软骨细胞(P2)以多角形为主,Ⅱ型胶原蛋白表达阳性,提示P2代细胞表型维持良好;与A组比较,B组细胞数量较多且分布均匀,细胞形态较均一,以多角形为主;A、B组样本大体结构及内部孔隙结构均保持完整,孔隙内均可见细胞黏附,B组孔隙内细胞数量及细胞外基质优于A组;A、B组的Ⅱ型胶原基因mRNA表达水平较单层培养组明显增加。[结论]软骨细胞复合Ⅰ型胶原支架体外动态培养较静态培养可明显促进细胞增殖及细胞外基质分泌。因此,动态培养有望成为体外构建自体软骨细胞移植术所需移植物的一种有效方法。

关键词(KeyWords)： 软骨细胞;Ⅰ型胶原支架材料;动态培养

Abstract:

Keywords:

基金项目(Foundation): 广东省自然科学基金项目(编号:S2012010008129);; 深圳市科技创新委技术攻关项目(编号:JSGG2014051905550503);; 深圳市科技创新委国际科技合作项目(编号:GJHZ20130412159306739);; 深圳市重点实验室提升项目(编号:CXB201104220049A);; 深圳市科技研发资金项目(编号:CXZZ20120614160234842;ZDSY20120614154551201);; 中国博士后科学基金面上项目(编号:2013M530385)

作者(Author): 王泽民;段莉;梁宇杰;王大平;

Email:

DOI:

参考文献(References)：

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