徐一宏;徐卫东;

• 1:海军军医大学附属长海医院关节骨病外科

摘要(Abstract)：

[目的]探讨二代测序技术在关节置换术后假体周围感染诊断中的应用价值。[方法]选取2018年1月～2018年12月在本院就诊的人工关节置换术后关节疼痛、红肿、怀疑假体周围感染的患者。依据美国肌肉、骨骼感染病学会(MSIS)诊断标准,最终共计22例患者确诊为人工关节置换术后假体周围感染。其中男10例,女12例;膝关节感染16例,髋关节感染6例。所有22例PJI患者均行关节液二代测序和细菌培养。分别记录二代测序及细菌培养结果,以检测或培养出微生物为阳性,未检测或培养出微生物为阴性,记录阳性例数。分别计算二代测序技术和细菌培养在PJI患者中的阳性检出率以及在髋膝不同部位的阳性检出率,并通过配对卡方检验进行统计学分析。[结果]总计22例PJI患者中,18例患者二代测序检出微生物,二代测序在PJI患者中的阳性检出率为18/22(81.82%);关节液细菌培养阳性9例,关节液细菌培养在PJI患者中的阳性检出率为9/22(40.91%)。配对卡方检验提示两种方法在PJI患者中的检出率差异有统计学意义(P=0.022)。[结论]二代测序技术在PJI患者的诊断中具有更高的细菌检出率,在PJI患者病原微生物学检查中有更大的诊断价值。

关键词(KeyWords)： 全关节置换;假体周围感染;二代测序;细菌培养;诊断

Abstract:

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作者(Author): 徐一宏;徐卫东;

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参考文献(References)：

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