刘效仿;张健;侯蕾;黄勋福;吴苑妍;刘礼初;

• 1:广东省佛山市中医院

摘要(Abstract)：

[目的]观察透明质酸对关节软骨损伤修复的作用,并探讨其机制。[方法]经手术建立兔股骨内髁软骨2.5 mm圆形缺损模型,术中于缺损处局部注射、术后每周关节内注射透明质酸0.5 m1。4周后组织学和免疫组织化学分析缺损处软骨修复情况及凋亡细胞发生率;并分析关节液中Ⅱ型胶原、粘多糖和肿瘤坏死因子含量。[结果]透明质酸对关节软骨缺损的修复有明显的促进作用,4周时组织学上可见纤维软骨修复为主,其间散在少量Ⅱ型胶原阳性细胞,部分纤维软骨向透明软骨转化。并且关节液中Ⅱ型胶原和粘多糖增多,TNF-α含量减少,同时软骨和软骨下骨中凋亡细胞发生率降低。[结论]局部注射透明质酸可能通过影响骨髓干细胞增殖与分化,自骨髓和软骨下骨向缺损处诱发新生软骨组织的形成,从而促进软骨损伤的修复。

关键词(KeyWords)： 透明质酸;关节软骨;缺损;修复

Abstract:

Keywords:

基金项目(Foundation): 广东省中医药局资助项目(编号:20123016);; 佛山市院外合作项目(创新载体建设)(编号:2014H10004)

作者(Author): 刘效仿;张健;侯蕾;黄勋福;吴苑妍;刘礼初;

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参考文献(References)：

• [1]Triche R,Mandelbaum BR.Overview of cartilage biology and new trends in cartilage stimulation[J].Foot Ankle Clin,2013,18(1):1-12.
• [2]Huey DJ,Hu JC,Athanasiou KA.Unlike bone,cartilage regeneration remains elusive[J].Science,2012,16(338):917-921.
• [3]Ahmed TA,Hincke MT.Strategies for articular cartilage lesion repair and functional restoration[J].Tissue Eng Part B Rev,2010,16(3):305-329.
• [4]Kessler MW,Ackerman G,Dines JS,et al.Emerging technologies and fourth generation issues in cartilage repair[J].Sports Med Arthrose,2008,16(4):246-254.
• [5]Curran MP.Hyaluronic acid(Supartz):a review of its use in osteoarthritis of the knee[J].Drugs Aging,2010,27(11):925-941.
• [6]Banu N,Tsuchiya T.Markedly different effects of hyaluronic acid and chondroitin sulfate-A on the differentiation of human articular chondrocytes in micromass and 3-D honeycomb rotation cultures[J].Biomed Mater Res A,2007,80(2):257-267.
• [7]Nettles DL,Vail TP,Morgan MT,et al.Photocrossl inkable hyaluronan as a scaffold for articular cartilage repair[J].Ann Biomed Eng,2004,32(3):391-397.
• [8]Liu PF,Guo L,Zhao DW,et al.Study of human acellular amniotic membrane loading bone marrow mesenchymal stem cells in repair of articular cartilagedefect in rabbits[J].Genet Mol Res,2014,13(3):7992-8001.
• [9]Liu LC,Aronsona J,Lecka-Czernik B.Rosiglitazone disrupts endosteal bone formation during distraction osteogenesis by local adipocytic infiltration[J].Bone,2013,52(1):2013:247-258.
• [10]Giorgi M,Carriero A,Shefelbine SJ,et al.Mechanobiological simulations of prenatal joint morphogenesis[J].Biomech,2014,47(5):989-995.
• [11]Eggli PS,Hermann W,Hunziker EB,et al.Matrix compartments in the growth plate of the proximal tibia of rats Anat Rec,1985,211(3):246-257.
• [12]Guilak F,Mow VC.The mechanical environment of the chondrocyte:a biphasic finite element model of cell-matrix interactions in articular cartilage[J].Biomech,2000,33(12):1663-1673.
• [13]Eyre DR,Weis MA,Wu JJ.Articular cartilage collagen:an irreplaceable framework[J].Eur Cell Mater,2006,2(12):57-63.
• [14]Lima EG,Bian L,Ng KW,et al.The beneficial effect of delayed compressive loading on tissue-engineered cartilage constructs cultured with TGF-beta3[J].Osteoarthritis Cartilage,2007,15(9):1025-1033.
• [15]Gudmann NS,Wang J,Hoielt S,et al.Cartilage turnover reflected by metabolic processing of typeⅡcollagen:a novel marker of anabolic function in chondrocytes[J].Int J Mol Sci,2014,15(10):18789-18803.