徐亦驰;尹合勇;孙振;孟昊业;肖波;余晓明;苑志国;汪爱媛;彭江;卢世璧;

• 1:中国人民解放军总医院骨科研究所骨科再生医学北京市重点实验室全军骨科战创伤重点实验室
• 2:中国人民解放军医学院
• 3:南开大学医学院

摘要(Abstract)：

[目的]通过Micro-CT图像和其数值数据评价并对比镁合金与PLGA在兔股骨髁内对新骨形成的促进作用。[方法]取体重2.2 kg、3个月龄的雄性新西兰大白兔30只,随机分为3组,每组10只,表面微弧氧化处理的AZ31镁合金棒材(以下简称"镁棒")30个和PLGA棒材30个分别置入到30只兔子的右、左侧股骨髁。置入后4、8、12周,3个时间点依次取出3组兔子的双侧股骨髁,利用Micro-CT扫描图像以及生成数据定量对镁合金和PLGA置入部位周围新骨生成情况进行评价和比较。[结果]在4周以前,骨生成活动并不十分活跃,两种材料置入周围的新骨骨体积分数无明显差异;在第8~12周,两组的新骨生成速度加快,尤其是AZ31组新生骨体积分数增长更为明显,且AZ31组的新骨骨体积分数明显高于PLGA组(P<0.05)。新生骨表面积与其体积之比(BS/BV)随着实验进展持续降低,在第8~12周时降低速度最快,且在第12周时AZ31周围新生骨BS/BV低于PLGA组出现统计学意义(P<0.05)。随着置入物的降解,两组新生骨均出现骨小梁厚度增加,数目增多,而间距减少,同样在第8~12周,AZ31组骨小梁厚度和数目的增长明显高于PLGA组(P<0.05),且3个时间点的AZ31镁合金组和PLGA组骨小梁间距减小差异均有统计学意义(P<0.05)。此外,实验过程中不仅骨量增加,新生骨组织矿物密度随着实验进展也在持续增加,但不同材料的两组间并无明显差异。[结论]AZ31镁合金在体内对新生骨生长的促进作用明显优于PLGA材料。

关键词(KeyWords)： Micro-CT;镁合金;PLGA;骨生成;体内实验

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(NFSC,编号:81572148,51361130034);; 国家重点基础研究发展计划(973计划,编号:2012CB518106);; 军队十二五发展计划(编号:BWS11J025);; 军队重点科学研究项目(编号:BWS13C029);; 北京市自然科学基金(编号:Z141107004414044)

作者(Author): 徐亦驰;尹合勇;孙振;孟昊业;肖波;余晓明;苑志国;汪爱媛;彭江;卢世璧;

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DOI:

参考文献(References)：

• [1]Staiger MP,Pietak AM,Huadmai J,et al.Magnesium and its alloys as orthopedic biomaterials:a review[J].Biomaterials,2006,27(9):1728-1734.
• [2]王富勇,陶海荣.骨科内固定材料镁合金的生物学研究进展[J].中国矫形外科杂志,2015,23(4):322-324.
• [3]Ratna Sunil B,Sampath Kumar TS,Chakkingal U,et al.In vitro and in vivo studies of biodegradable fine grained AZ31 magnesium alloy produced by equal channel angular pressing[J].Mater Sci Eng C Mater Biol Appl,2016,59:356-367.
• [4]于国宁,潘锋,闻久全,等.镁合金体内植入生物安全性的初步研究[J].中国矫形外科杂志,2008,16(13):1015-1018.
• [5]Castellani C,Lindtner RA,Hausbrandt P,et al.Bone-implant interface strength and osseointegration:biodegradable magnesium alloy versus standard titanium control[J].Acta Biomater,2011,7(1):432-440.
• [6]Han P,Tan M,Zhang S,et al.Shape and site dependent in vivo degradation of Mg-Zn pins in rabbit femoral condyle[J].Int J Mol Sci,2014,15(2):2959-2970.
• [7]Kraus T,Fischerauer SF,Hnzi AC,et al.Magnesium alloys for temporary implants in osteosynthesis:in vivo studies of their degradation and interaction with bone[J].Acta Biomater,2012,8(3):1230-1238.
• [8]王树峰,李春荣,王程越,等.微弧氧化AZ31镁合金的生物相容性[J].中国组织工程研究,2012,16(38):7101-7106.
• [9]Lindtner RA,Castellani C,Tangl S,et al.Comparative biomechanical and radiological characterization of osseointegration of a biodegradable magnesium alloy pin and a copolymeric control for osteosynthesis[J].J Mech Behav Biomed Mater,2013,28(28c):232-243.
• [10]Brown A,Zaky S,Ray H Jr,et al.Porous magnesium/PLGA composite scaffolds for enhanced bone regeneration following tooth extraction[J].Acta Biomater,2015,11(1):543-553.
• [11]Liu H,Wang R,Chu HK,et al.Design and characterization of a conductive nanostructured polypyrrole-polycaprolactone coated magnesium/PLGA composite for tissue engineering scaffolds[J].J Biomed Mater Res A,2015,103(9):2966-2973.
• [12]薛静,彭江,汪爱媛,等.活体小动物Micro-CT动态评价大鼠股骨牵张成骨[J].中国矫形外科杂志,2010,18(9):752-758.
• [13]彭江,汪爱媛,孙明学,等.Micro-CT在松质骨结构研究中的应用[J].中国矫形外科杂志,2005,13(11):859-861.
• [14]Clark DP,Badea CT.Micro-CT of rodents:State-of-the-art and future perspectives[J].Physica Med,2014,30(6):619-634.
• [15]Schambach SJ,Bag S,Schilling L,et al.Application of micro-CT in small animal imaging[J].Methods,2010,50(1):2-13.
• [16]Fischerauer SF,Kraus T,Wu X,et al.In vivo degradation performance of micro-arc-oxidized magnesium implants:a micro-CT study in rats[J].Acta Biomater,2013,9(2):5411-5420.
• [17]张涛,武肖娜,尹庆水,等.镁合金AZ31B材料表性与成骨细胞的黏附[J].中国组织工程研究,2013,17(12):2123-2130.
• [18]齐峥嵘,张强,殷毅,等.可降解镁合金作为骨植入材料的体内研究进展[J].中国修复重建外科杂志,2012,26(11):1381-1386.
• [19]Brooks EK,Der S,Ehrensberger MT.Corrosion and mechanical performance of AZ91 exposed to simulated inflammatory conditions[J].Mater Sci Eng C Mater Biol Appl,2016,60(3):427-436.
• [20]Zhao N,Zhu DH.Collagen self-assembly on orthopedic magnesium biomaterials surface and subsequent bone cell attachment[J].PLo S ONE,9(10):e110420.
• [21]Grünewald TA,Ogier A,Akbarzadeh J,et al.Reaction of bone nanostructure to a biodegrading magnesium WZ21 implant-a scanning small-angle X-ray scattering time study[J].Acta Biomater,2016,31:448-457.
• [22]Vladimirov BV,Krit BL,Lyudin VB,et al.Microarc oxidation of magnesium alloys:a review[J].Surface Eng App Elect,2014,50(3):195-232.
• [23]Walker J,Shadanbaz S,Woodfield TB,et al.Magnesium biomaterials for orthopedic application:a review from a biological perspective[J].J Biomed Mater Res B Appl Biomater,2014,102(6):1316-1331.
• [24]Meininger S,Mandal S,Kumar A,et al.Strength reliability and in vitro degradation of three-dimensional powder printed strontiumsubstituted magnesium phosphate scaffolds[J].Acta Biomater,2015,31:401-411.
• [25]Choudhary L,Raman S.Magnesium alloys as body implants:Fracture mechanism under dynamic and static loadings in a physiological environment[J].Acta Biomater,2012,8(2):916-923.
• [26]Witte F.The history of biodegradable magnesium implants:a review[J].Acta Biomater,2011,6(5):1680-1692.
• [27]张佳,宗阳,付彭怀,等.镁合金在生物医用材料领域的应用及发展前景[J].中国组织工程研究与临床康复,2009,13(29):547-575.
• [28]苗波,姜德志.医用镁合金微弧氧化后不同涂层抑菌性及骨诱导性的研究[J].黑龙江医药科学,2009,32(5):7-8.
• [29]Vanderoost J,Lenthe GH.From histology to micro-CT:Measuring and modeling resorption cavities and their relation to bone competence[J].World J Radio,2014,6(9):643-656.
• [30]Pereira RC,Bischoff DS,Yamaguchi D,et al.Micro-CT in the assessment of pediatric renal osteodystrophy by bone histomorphometry[J].Clin J Am Soc Nephrol,2016,11(3):481-487.
• [31]Daoust A,Barbier EL,Bohic S.Manganese enhanced MRI in rat hippocampus:a correlative study with synchrotron X-ray microprobe[J].Neuroimage,2013,64(1):10-18.