王文;秦胜男;陈鸿辉;董飞;李爱国;沈雁;

• 1:暨南大学附属广州市红十字会医院骨科、创伤外科研究所

摘要(Abstract)：

[目的]观察白藜芦醇对人肌腱干细胞成骨分化的作用及其细胞信号通路。[方法]低密度接种法和胶原酶消化法分离培养人肌腱干细胞,结晶紫染色观察分离出的细胞形成的单克隆,体外诱导检测分离细胞的多向分化能力;在/无成骨诱导因子下,不同浓度白藜芦醇刺激人肌腱干细胞荧光定量PCR检测骨相关基因(Run x2和OCN)表达,免疫印迹检测Run x2蛋白表达,茜素红S染色观察钙盐沉积,免疫印迹检测β-catenin和NK-KB表达以检测相关细胞信号通路。[结果]成功从人髌腱组织中分离培养出肌腱干细胞,体外可被诱导成骨细胞和脂肪细胞;在/无成骨诱导因子影响下,白藜芦醇均促进骨相关因子Run x2和其下游因子OCN表达,且随着白藜芦醇的浓度升高而上调;无成骨诱导因子影响下,Run x2蛋白表达随着白藜芦醇浓度升高而增多,而在成骨诱导因子存在下,其蛋白表达变化不明显。在/无成骨诱导因子影响下,茜素红S染色显示白藜芦醇促进人肌腱干细胞的钙盐沉积。随着白藜芦醇浓度的提高,β-catenin表达减少,而NF-KB表达无明显变化。[结论]白藜芦醇可以体外诱导并促进人肌腱干细胞的成骨分化,可能通过调节β-catenin信号通路来调控。

关键词(KeyWords)： 白藜芦醇;β-catenin;人肌腱干细胞;成骨分化

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(编号:30973067,81228013);; 广东省中医药局项目(编号:20111001);; 广州市卫生局重点项目(编号:201102A212005,201102A212003);; 广州市卫生局一般引导项目(编号:2012A001052);; 广州市中西医结合项目(编号:2012A011015)

作者(Author): 王文;秦胜男;陈鸿辉;董飞;李爱国;沈雁;

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DOI:

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