脊髓损伤后线粒体超微结构和呼吸功能的变化Changes of ultrastructures and respiratory function of the spinal cord mitochondrial in experimental spinal cord injury
蔡卫华;张宁;贾连顺;叶晓健;殷国勇;叶添文;
摘要(Abstract)：
[目的]探讨大鼠脊髓损伤后伤段脊髓线粒体超微结构的改变和线粒体呼吸功能的变化。[方法]48只SD大鼠,随机分为:对照组(假手术组)和脊髓损伤组(SCI组),每组又分为处理后6、12、24 h三时相组,每组8只,采用A llen's打击法造成大鼠脊髓损伤模型,在各时相提取伤段脊髓线粒体,透射电镜观察线粒体超微结构的改变;C lark氧电极法测定线粒体呼吸功能,根据线粒体呼吸耗氧量换算出R3、R4、RCR、P/O比值。[结果]SC I组脊髓组织线粒体体积增大,嵴稍肿胀,嵴内腔扩大,部分线粒体出现膜结构不清、嵴紊乱,部分神经细胞内的高尔基器、内质网稍肿大。随着损伤后时间的延长,可见部分线粒体嵴断裂溶解、膜破裂和线粒体数量减少等。SC I组在伤后6、12 h和24 h R3、RCR和P/O显著低于对照组,R4显著高于对照组,有极显著统计学意义(P<0.01)。[结论]大鼠脊髓损伤后线粒体的超微结构发生明显改变;伤段脊髓线粒体呼吸功能明显下降。
关键词(KeyWords)： 脊髓损伤;线粒体;超微结构
基金项目(Foundation): 军队医药卫生科研基金资助项目(项目编号:01MB101)
作者(Author): 蔡卫华;张宁;贾连顺;叶晓健;殷国勇;叶添文;
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