石晓伟,黄亮亮,夏冰,高楗勃,杨雨洁,罗卓荆,黄景辉

• 1:空军军医大学西京医院骨科

摘要(Abstract)：

[目的]制备可控释神经生长因子(nerve growth factor, NGF)的阵列微管神经修复支架,并评价其促神经再生效能。[方法]采用梯度冷凝技术制备复合NGF的阵列微管神经修复支架,检测其机械特性和NGF释放规律。将新生SD大鼠的背根神经节(dorsal root ganglions, DRG)随机分为4组:无序支架组、复合NGF的无序支架组、阵列微管支架组和复合NGF的阵列微管支架组。将DRG植入各组支架中体外培养72 h,观察轴突生长情况。[结果]在机械强度方面,各组在横向压缩过程中的峰值载荷差异无统计学意义(P>0.05)。纵向牵拉测试中,神经支架组与自体神经组的应力-应变曲线类似。在NGF释放规律方面,阵列微管组和无序组的释放曲线类似,且不同时间点NGF浓度差异无统计意义(P>0.05)。在轴突生长方面,复合NGF的阵列微管支架组轴突长度为(1 025.41±175.33)μm,显著高于阵列微管支架组(857.58±233.14)μm、复合NGF无序支架组(341.73±52.27)μm、无序支架组(225.36±61.62)μm。[结论]复合NGF的阵列微管神经修复支架同时具有营养活性及引导结构,且具有良好的机械性能,在体外环境下可以引导大鼠DRG轴突定向延伸,在周围神经再生领域具有重要前景。

关键词(KeyWords)： 丝素蛋白;神经生长因子;阵列微管;神经支架;背根神经节

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(编号:81672148;81201389);; 国家重点研发计划项目(编号:2016YFC1101700);; 国家重点基础研究项目(973计划,编号:2014CB542206);; 长江学者和创新发展团队计划项目(编号:IRT1053;IRT13051);; 全国优秀博士论文基金项目(编号:201480)

作者(Author): 石晓伟,黄亮亮,夏冰,高楗勃,杨雨洁,罗卓荆,黄景辉

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