秦胜男;陈鸿辉;杨小红;康宁;谭见容;梁佩红;戴丽冰;叶春婷;

• 1:暨南大学附属广州市红十字会医院广州市创伤外科研究所

摘要(Abstract)：

[目的]用碳化二亚胺[1-ethyl-3-(3-dimethyl ami-nopropyl)carbodiimide,EDC]/N-羟基琥珀酰亚胺(N-hydroxysuccinimide,NHS)对Ⅱ型胶原海绵进行交联,评价了交联前后材料理化性能的改变,探讨其在软骨组织工程方面应用的可行性。[方法]提取Ⅱ型胶原后,通过真空冷冻干燥法冻干成多孔海绵材料后,室温下交联24h,再次冻干成为Ⅱ型胶原海绵支架材料。采用激光共聚焦显微镜、扫描电镜、拉力测试机等手段对海绵材料的结构及性能进行分析。将绿色荧光蛋白(GFP)标记的兔软骨细胞种植到交联后的Ⅱ型胶原支架材料后,观察细胞生长状况。[结果](1)交联后的海绵材料的力学性能与未交联组相比明显提高,达到(2.18±0.47)MPa;(2)交联组材料的抗胶原酶降解能力显著提高,4h后仅降解了8.28%。交联后孔径大小约为90μm,孔隙率和平衡含水率平均为93.39%和97.78%;(3)软骨细胞在Ⅱ型胶原海绵上生长状况良好。[结论]交联后的Ⅱ型胶原海绵保持其优良的理化特性和生物相容性,同时又有效地提高了力学性能和抗降解能力。

关键词(KeyWords)： Ⅱ型胶原;碳化二亚胺;N-羟基琥珀酰亚胺;交联

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(编号:C160705);; 广州市科技攻关项目(编号:200623-E0371);; 广州医药卫生科技重点项目(编号:2006-Zdi-03,2006-ZDi-04)

作者(Author): 秦胜男;陈鸿辉;杨小红;康宁;谭见容;梁佩红;戴丽冰;叶春婷;

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