贾海栋,李毅

• 1:邯郸市中心医院

摘要(Abstract)：

[目的]探讨兔脂肪间充质干细胞膜片对兔关节软骨损伤的修复作用。[方法]体外分离培养兔脂肪间充质干细胞，通过多系分化对细胞进行鉴定。通过化学方法构建兔脂肪间充质干细胞膜片（添加50μg/ml抗坏血酸），膜片构建成功后对其进行检测：分别在倒置相差显微镜下观察膜片的结构，大体观以及扫描电镜观察。制备兔膝关节骨软骨缺损模型（直径为3.5mm，深度为3 mm），然后将实验分为两组：对照组和细胞膜片组。术后3个月取标本进行检测，观察软骨的修复情况。[结果]原代兔脂肪间充质干细胞呈短梭形、三角形贴壁生长，经过传代培养后细胞逐渐呈涡旋状生长，第3代兔脂肪间充质干细胞具有多系分化能力；兔脂肪间充质干细胞膜片具有多层结构，细胞分泌大量的细胞外基质，将细胞镶嵌在其中。术后3个月取标本观察检测提示：细胞膜片组骨软骨缺损处可见大量新生的软骨组织再生，新生的软骨组织形态和颜色均接近于正常软骨；而对照组几乎没有新生软骨的形成。O’Driscoll评分结果也显示细胞膜片组的评分明显高于对照组，差异有统计学意义（P<0.05）。[结论]兔脂肪间充质干细胞膜片能够促进兔关节软骨的再生，为临床软骨损伤的修复提供了新的思路。

关键词(KeyWords)： 脂肪间充质干细胞膜片;关节软骨;组织工程

Abstract:

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基金项目(Foundation):

作者(Author): 贾海栋,李毅

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