曲峰,袁邦拓,齐玮,李春宝,王俊良,申学振,王江涛,肇刚,刘玉杰

• 1:解放军总医院骨科运动医学中心

摘要(Abstract)：

[目的]探讨Wnt3a基因对骨髓间充质干细胞(BMSCs)成软骨分化的影响。[方法]分离培养大鼠骨髓间充质干细胞(BMSCs),携带Wnt3a及阴性对照基因(Mock)的慢病毒感染骨髓间充质干细胞,构建过表达Wnt3a骨髓间充质干细胞;采用甲苯胺蓝染色及QPCR观察Wnt3a对骨髓间充质干细胞成软骨分化能力的影响;使用MTT法观察Wnt3a对骨髓间充质干细胞增殖能力的影响。[结果]在0~72 h内,实验组MTT法检测吸光值明显大于对照组(P<0.05);BMSCs体外成软骨诱导后,实验组细胞团明显小于对照组,且甲苯胺蓝染色较浅;实验组与对照组比较,成软骨指标Col2A1、Aggrecan和SOX9的m RNA表达降低(P<0.05)。[结论]Wnt3a可促进骨髓间充质干细胞增殖,但抑制了骨髓间充质干细胞的成软骨分化。

关键词(KeyWords)： Wnt3a;骨髓间充质干细胞;成软骨分化

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(编号:81271983)

作者(Author): 曲峰,袁邦拓,齐玮,李春宝,王俊良,申学振,王江涛,肇刚,刘玉杰

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